The rs9402373 polymorphism of CTGF gene may not be related to inflammatory bowel disease susceptibility in Chinese population based on ARMS-PCR genotyping

BackgroundIt has been confirmed that the connective tissue growth factor (CTGF) gene rs9402373 polymorphism is associated with fibrotic and inflammatory diseases. However, studies on relationship between polymorphisms in CTGF bowel disease (IBD) remain rare. Therefore, aim of this study was to assess association IBD susceptibility a Chinese population.Materials methodsTo establish an amplification refractory mutation system (ARMS) PCR technology for genotyping polymorphism, we designed two specific forward primers wild mutant types by placing allele-specific nucleotide at penultimate position ‘3′ end primer. Then, 10 samples were randomly selected rechecked DNA sequencing verify accuracy method. We further used established method detect specimens collected from 191 patients disease, including 120 Crohn's (CD) 71 ulcerative colitis (UC), 110 healthy Han individuals.ResultsWe successfully ARMS-PCR genotyping, results completely consistent sequencing. The G allele frequencies UC CD cases 38.03% 43.75%, respectively, controls, they 41.82%. No significant difference found minor or control groups (P = 0.473, P 0.676). Genotype analysis demonstrated there no risk regardless inheritance mode > 0.05).ConclusionsIn preliminary study, developed simple, efficient cost-effective polymorphism. may not be related population.